paramedicpete, I was wondering if you have time down the road to try your steripen out with those test and see if you get the same,worst or better results. And compare the time, the 1 1/2 minute vs the Miox 4 hour time. In my case I would boil my water than wait on the time for a miox. That's why I had bought my steripen. Any how if you are game in testing and comparing it would be appreciated.
Thanks
And from Arney: I suspect that it wouldn't be practical. In Pete's original protocol, he said, "The bacterial slurry was significantly turbid to the naked eye." So, that would rule out using the Steripen with the same protocol since the Steripen is designed to be used in clear water. He could use a different solution, but then it wouldn't be directly comparable with the other results.
Actually, I did (sort of- only tested with one species of bacteria- I think), the post is so old it is not longer available on the site.
IIRC, I made turbid slurry of E. coli, post 1 round of the Steri-pen there was a substantial reduction in the number of organisms. It took two rounds of the Steri-pen to achieve 0 cfu (colony forming units). The turbidity created from the concentrated organism is different then turbidity from say, slit or mud. Keep in mind when using the Steri-pen, you are supposed to “mix” or move the pen around the container (like mixing with a spoon), this exposes most of the water more directly to the UV source. With the bacteria slurry, although turbid, the mixing caused the individual bacteria to come close to the UV source, thus killing the organism. With turbidity caused by silt, the silt might block the UV from hitting the individual bacteria; the “blocking” effect would likely be more pronounced with small bacteria and viruses, which might be small enough to “hide” in the rough surface of the individual silt particle
I hope this explanation makes sense.
Pete