I think the moment that the water reaches its boiling point, technically whatever you're trying to kill is dead. The reason you're told to boil it is just so there's no guessing if the water is hot enough... If I recall correctly...

I read that in an thread here in the forums. Can anyone back this up, or have I lost my marbles?

In any case, I'd probably let it go for a couple of minutes at a boil because I'm paranoid.